Abstract:
Detection of microbial contamination by plating methods, especially coliform
bacteria and other pathogens, many still use conventional methods and take about 7 days to
find out the results. RTU (ready to use) media is one of the selective media to determine
microbes more quickly based on the color of the colonies on the agar media. This study used
RTU media for determination of biosafety of Mi Lethek. Mi Lethek, is cassava noodles
originated from Bendo, Srandakan, Bantul, Yogyakarta, made from cassava starch and cassava
flour. Production of Lethek noodles uses traditional machinery and equipments. Biosafety
analysis was performed on dried mie lethek. The presence of enteric bacteria is known by the
presence of blue colonies for Eschericia coli and purple/magenta for Salmonella. The results
showed that microbial contamination in dried Lethek noodles was less than 10
cfu/g.
Contamination of enteric bacteria was less than 10
2
cfu/10g. Indonesian standard (SNI) of dried
noodles was 10
6
cfu/g for total of microbial and 10 cfu/g for enteric bacteria (E. coli). Although
mie lethek quality complies SNI quality standards, sanitation should be improved.
